Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2537043..2537540 Replicon chromosome
Accession NZ_CP053258
Organism Escherichia coli strain GF4-3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HLY11_RS11900 Protein ID WP_001173343.1
Coordinates 2537043..2537216 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HLY11_RS11905 Protein ID WP_000398619.1
Coordinates 2537244..2537540 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY11_RS11890 2533713..2535170 + 1458 WP_059320083.1 dipeptide/tripeptide permease DtpC -
HLY11_RS11895 2535407..2536924 + 1518 WP_001295074.1 lysine--tRNA ligase -
HLY11_RS11900 2537043..2537216 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HLY11_RS11905 2537244..2537540 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HLY11_RS11910 2537767..2538039 - 273 WP_000405647.1 N-acetyltransferase -
HLY11_RS11915 2538051..2538281 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
HLY11_RS11920 2538462..2540093 + 1632 WP_001216477.1 sensor histidine kinase -
HLY11_RS11925 2540090..2540809 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T156466 WP_001173343.1 NZ_CP053258:c2537216-2537043 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T156466 NZ_CP069065:c2351442-2351008 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT156466 WP_000398619.1 NZ_CP053258:c2537540-2537244 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT156466 NZ_CP069065:c2351703-2351449 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References