Detailed information of TA system TA156465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2426624..2427219
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: HLY11_RS11360
• Protein ID: WP_171459924.1
• Coordinates: 2426624..2426974 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A240D6C8
• Locus tag: HLY11_RS11365
• Protein ID: WP_001223212.1
• Coordinates: 2426968..2427219 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS11340	2422073..2423095	-	1023	WP_001550507.1	ABC transporter permease	-
HLY11_RS11345	2423109..2424611	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
HLY11_RS11350	2424751..2425707	-	957	WP_171459923.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HLY11_RS11355	2426017..2426547	+	531	WP_000055075.1	inorganic diphosphatase	-
HLY11_RS11360	2426624..2426974	-	351	WP_171459924.1	endoribonuclease toxin ChpB	Toxin
HLY11_RS11365	2426968..2427219	-	252	WP_001223212.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HLY11_RS11370	2427430..2427771	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HLY11_RS11375	2427774..2431553	-	3780	WP_171459925.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12506.43 Da
• Isoelectric Point: 5.5572

>T156465 WP_171459924.1 NZ_CP053258:c2426974-2426624 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLLAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T156465 NZ_CP069065:2310137-2310547 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9301.61 Da
• Isoelectric Point: 4.6509

>AT156465 WP_001223212.1 NZ_CP053258:c2427219-2426968 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT156465 NZ_CP069065:2309911-2310144 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A240D6C8