Detailed information of TA system TA156464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2415149..2415671
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HLY11_RS11300
• Protein ID: WP_171459922.1
• Coordinates: 2415149..2415439 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: HLY11_RS11305
• Protein ID: WP_000212715.1
• Coordinates: 2415429..2415671 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS11285	2410339..2411994	+	1656	WP_171459921.1	alpha,alpha-phosphotrehalase	-
HLY11_RS11290	2412388..2414526	+	2139	WP_039022460.1	anaerobic ribonucleoside-triphosphate reductase	-
HLY11_RS11295	2414684..2415148	+	465	WP_001106233.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HLY11_RS11300	2415149..2415439	-	291	WP_171459922.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HLY11_RS11305	2415429..2415671	-	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HLY11_RS11310	2415862..2416248	-	387	WP_001232245.1	cytochrome b562	-
HLY11_RS11315	2416431..2417783	-	1353	WP_112844257.1	metalloprotease PmbA	-
HLY11_RS11320	2417877..2418428	+	552	WP_000166270.1	ribosome-associated protein	-
HLY11_RS11325	2418511..2419884	-	1374	WP_001219814.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11382.29 Da
• Isoelectric Point: 10.0238

>T156464 WP_171459922.1 NZ_CP053258:c2415439-2415149 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDLLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYQVNDKELLVLVISIGK
RENSAVYEDATKRLDE

Nucleotide

• Length: 291 bp

>T156464 NZ_CP069065:2242355-2242753 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT156464 WP_000212715.1 NZ_CP053258:c2415671-2415429 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT156464 NZ_CP069065:2242127-2242354 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3