Detailed information of TA system TA156463

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2363631..2364338
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HLY11_RS11075
• Protein ID: WP_171459900.1
• Coordinates: 2363631..2363972 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: HLY11_RS11080
• Protein ID: WP_000939437.1
• Coordinates: 2364003..2364338 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS11050	2359262..2360242	+	981	WP_171459898.1	sialate O-acetylesterase	-
HLY11_RS11055	2360250..2360372	-	123	WP_171459899.1	HNH endonuclease	-
HLY11_RS11060	2361278..2362225	-	948	WP_032251793.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HLY11_RS11065	2362218..2362613	-	396	WP_000152739.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HLY11_RS11070	2362683..2363516	-	834	WP_130076282.1	DUF4942 domain-containing protein	-
HLY11_RS11075	2363631..2363972	-	342	WP_171459900.1	TA system toxin CbtA family protein	Toxin
HLY11_RS11080	2364003..2364338	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HLY11_RS11085	2364338..2364811	-	474	WP_001292743.1	DNA repair protein RadC	-
HLY11_RS11090	2364841..2365659	-	819	WP_171459901.1	DUF932 domain-containing protein	-
HLY11_RS11095	2365896..2366849	-	954	WP_171459902.1	hypothetical protein	-
HLY11_RS11100	2367442..2368056	+	615	WP_171459903.1	inovirus-type Gp2 protein	-
HLY11_RS11105	2368174..2368392	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2354407..2381835	27428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13104.13 Da
• Isoelectric Point: 9.4583

>T156463 WP_171459900.1 NZ_CP053258:c2363972-2363631 [Escherichia coli]
MKIIPATTLRATTSYLSPIVVWQTLLTRLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T156463 NZ_CP069065:c2218738-2218394 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT156463 WP_000939437.1 NZ_CP053258:c2364338-2364003 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT156463 NZ_CP069065:c2218980-2218732 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6