Detailed information of TA system TA156461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2286581..2287157
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HLY11_RS10845
• Protein ID: WP_171459872.1
• Coordinates: 2286870..2287157 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: HLY11_RS10840
• Protein ID: WP_000063156.1
• Coordinates: 2286581..2286883 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS10820	2282342..2282884	+	543	WP_000934391.1	hypothetical protein	-
HLY11_RS10825	2283177..2285327	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
HLY11_RS10830	2285377..2285580	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HLY11_RS10835	2285591..2286547	+	957	WP_001295525.1	GTPase	-
HLY11_RS10840	2286581..2286883	-	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
HLY11_RS10845	2286870..2287157	-	288	WP_171459872.1	BrnT family toxin	Toxin
HLY11_RS10850	2287312..2288046	-	735	WP_171459873.1	Fic family protein	-
HLY11_RS10855	2288090..2290189	-	2100	WP_171459874.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.59 Da
• Isoelectric Point: 7.4697

>T156461 WP_171459872.1 NZ_CP053258:c2287157-2286870 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDLQHLSQQDRIENGEYRWQTIGVVHGIVVILAAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T156461 NZ_CP069065:c2209921-2209502 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT156461 WP_000063156.1 NZ_CP053258:c2286883-2286581 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT156461 NZ_CP069065:c2210190-2209930 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829G8Z0