Detailed information of TA system TA156459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1781778..1782615
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: A0A080JA52
• Locus tag: HLY11_RS08480
• Protein ID: WP_032170811.1
• Coordinates: 1782073..1782615 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A0A080JFU1
• Locus tag: HLY11_RS08475
• Protein ID: WP_001577859.1
• Coordinates: 1781778..1782089 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS08450	1776798..1777745	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HLY11_RS08455	1777767..1779758	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HLY11_RS08460	1779748..1780362	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HLY11_RS08465	1780362..1780691	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HLY11_RS08470	1780703..1781593	+	891	WP_000971336.1	heme o synthase	-
HLY11_RS08475	1781778..1782089	+	312	WP_001577859.1	DUF1778 domain-containing protein	Antitoxin
HLY11_RS08480	1782073..1782615	+	543	WP_032170811.1	GNAT family N-acetyltransferase	Toxin
HLY11_RS08485	1782671..1783606	-	936	WP_171459724.1	sel1 repeat family protein	-
HLY11_RS08490	1784013..1785377	+	1365	WP_001000978.1	MFS transporter	-
HLY11_RS08495	1785505..1785996	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HLY11_RS08500	1786164..1787075	+	912	WP_171459725.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19835.05 Da
• Isoelectric Point: 8.3395

>T156459 WP_032170811.1 NZ_CP053258:1782073-1782615 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRTSLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T156459 NZ_CP069065:2198526-2198903 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11400.08 Da
• Isoelectric Point: 10.5548

>AT156459 WP_001577859.1 NZ_CP053258:1781778-1782089 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNRLREAAQAVGLDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT156459 NZ_CP069065:2198945-2199394 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Toxin

• AlphaFold DB: A0A080JA52

Antitoxin

• AlphaFold DB: A0A080JFU1