Detailed information of TA system TA156457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1350061..1350766
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HLY11_RS06540
• Protein ID: WP_000539521.1
• Coordinates: 1350061..1350447 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HLY11_RS06545
• Protein ID: WP_001280945.1
• Coordinates: 1350437..1350766 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS06520	1346065..1346691	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HLY11_RS06525	1346688..1347803	-	1116	WP_171459593.1	aldose sugar dehydrogenase YliI	-
HLY11_RS06530	1347914..1348297	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HLY11_RS06535	1348510..1349835	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HLY11_RS06540	1350061..1350447	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLY11_RS06545	1350437..1350766	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HLY11_RS06550	1350836..1352164	-	1329	WP_171459594.1	GGDEF domain-containing protein	-
HLY11_RS06555	1352172..1354520	-	2349	WP_171459595.1	EAL domain-containing protein	-
HLY11_RS06560	1354698..1355609	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T156457 WP_000539521.1 NZ_CP053258:1350061-1350447 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T156457 NZ_CP069065:1956705-1956953 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT156457 WP_001280945.1 NZ_CP053258:1350437-1350766 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT156457 NZ_CP069065:1956493-1956705 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6