Detailed information of TA system TA156455

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 990744..991528
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HLY11_RS04800
• Protein ID: WP_053263446.1
• Coordinates: 991034..991528 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: HLY11_RS04795
• Protein ID: WP_001110446.1
• Coordinates: 990744..991037 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS04785	985893..986852	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
HLY11_RS04790	987425..990610	+	3186	WP_171459501.1	ribonuclease E	-
HLY11_RS04795	990744..991037	+	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
HLY11_RS04800	991034..991528	+	495	WP_053263446.1	GNAT family N-acetyltransferase	Toxin
HLY11_RS04805	991623..992576	-	954	WP_171459502.1	flagellar hook-associated protein FlgL	-
HLY11_RS04810	992588..994231	-	1644	WP_171459503.1	flagellar hook-associated protein FlgK	-
HLY11_RS04815	994297..995238	-	942	WP_040064085.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HLY11_RS04820	995238..996335	-	1098	WP_000589315.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18065.97 Da
• Isoelectric Point: 8.2617

>T156455 WP_053263446.1 NZ_CP053258:991034-991528 [Escherichia coli]
MIQAPEPLLARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T156455 NZ_CP069065:1768459-1768863 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT156455 WP_001110446.1 NZ_CP053258:990744-991037 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT156455 NZ_CP069065:1768250-1768453 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775