Detailed information of TA system TA156454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 686082..686720
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T4J978
• Locus tag: HLY11_RS03360
• Protein ID: WP_001612987.1
• Coordinates: 686544..686720 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HLY11_RS03355
• Protein ID: WP_001270286.1
• Coordinates: 686082..686498 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS03335	681234..682175	-	942	WP_171459415.1	ABC transporter permease	-
HLY11_RS03340	682176..683189	-	1014	WP_171459416.1	ABC transporter ATP-binding protein	-
HLY11_RS03345	683207..684352	-	1146	WP_171459417.1	ABC transporter substrate-binding protein	-
HLY11_RS03350	684597..686003	-	1407	WP_069913499.1	PLP-dependent aminotransferase family protein	-
HLY11_RS03355	686082..686498	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HLY11_RS03360	686544..686720	-	177	WP_001612987.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HLY11_RS03365	686942..687172	+	231	WP_000494244.1	YncJ family protein	-
HLY11_RS03370	687264..689225	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HLY11_RS03375	689298..689834	-	537	WP_000429144.1	DNA-binding transcriptional regulator SutR	-
HLY11_RS03380	689887..691098	+	1212	WP_171459418.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6725.78 Da
• Isoelectric Point: 10.9223

>T156454 WP_001612987.1 NZ_CP053258:c686720-686544 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKVRFHGMRSVMPRHPCDEIKEPLRKAIQKQLGLS

Nucleotide

• Length: 177 bp

>T156454 NZ_CP069065:1687891-1688208 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT156454 WP_001270286.1 NZ_CP053258:c686498-686082 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT156454 NZ_CP069065:1687592-1687894 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCTGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• AlphaFold DB: A0A3T4J978