Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4564768..4565422 Replicon chromosome
Accession NZ_CP053251
Organism Escherichia coli strain SCU-204

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ42_RS22100 Protein ID WP_000244781.1
Coordinates 4564768..4565175 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ42_RS22105 Protein ID WP_000354046.1
Coordinates 4565156..4565422 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ42_RS22080 4560725..4562458 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
HHJ42_RS22085 4562464..4563174 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ42_RS22090 4563199..4564095 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ42_RS22095 4564207..4564728 + 522 WP_001055874.1 flavodoxin FldB -
HHJ42_RS22100 4564768..4565175 - 408 WP_000244781.1 protein YgfX Toxin
HHJ42_RS22105 4565156..4565422 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ42_RS22110 4565665..4566645 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
HHJ42_RS22115 4566722..4567381 - 660 WP_000250274.1 hemolysin III family protein -
HHJ42_RS22120 4567545..4567856 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
HHJ42_RS22125 4567901..4569334 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T156439 WP_000244781.1 NZ_CP053251:c4565175-4564768 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156439 NZ_CP069065:708445-708846 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156439 WP_000354046.1 NZ_CP053251:c4565422-4565156 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156439 NZ_CP069065:708221-708448 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References