Detailed information of TA system TA156416

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1037600..1038432
• Replicon: chromosome
• Accession: NZ_CP053251
• Organism: Escherichia coli strain SCU-204

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: HHJ42_RS04855
• Protein ID: WP_000854753.1
• Coordinates: 1037600..1037974 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: HHJ42_RS04860
• Protein ID: WP_001540478.1
• Coordinates: 1038064..1038432 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ42_RS04835	1033953..1035491	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
HHJ42_RS04840	1036240..1036809	-	570	WP_001560692.1	DUF4942 domain-containing protein	-
HHJ42_RS04845	1036906..1037103	-	198	WP_000839281.1	DUF957 domain-containing protein	-
HHJ42_RS04850	1037115..1037603	-	489	WP_000777545.1	hypothetical protein	-
HHJ42_RS04855	1037600..1037974	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
HHJ42_RS04860	1038064..1038432	-	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ42_RS04865	1038595..1038816	-	222	WP_000692311.1	DUF987 domain-containing protein	-
HHJ42_RS04870	1038879..1039355	-	477	WP_001186786.1	RadC family protein	-
HHJ42_RS04875	1039371..1039856	-	486	WP_000214398.1	antirestriction protein	-
HHJ42_RS04880	1039947..1040765	-	819	WP_001773857.1	DUF945 domain-containing protein	-
HHJ42_RS04885	1040855..1041088	-	234	WP_001278283.1	DUF905 family protein	-
HHJ42_RS04890	1041094..1041771	-	678	WP_001097301.1	hypothetical protein	-
HHJ42_RS04895	1041919..1042599	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1026140..1085020	58880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T156416 WP_000854753.1 NZ_CP053251:c1037974-1037600 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T156416 NZ_CP069064:c2862077-2861682 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT156416 WP_001540478.1 NZ_CP053251:c1038432-1038064 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT156416 NZ_CP069064:c2862319-2862074 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68