Detailed information of TA system TA156414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4916980..4917618
• Replicon: chromosome
• Accession: NZ_CP053247
• Organism: Escherichia coli strain SCU-482

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: HHJ51_RS23735
• Protein ID: WP_000813797.1
• Coordinates: 4917442..4917618 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HHJ51_RS23730
• Protein ID: WP_076611057.1
• Coordinates: 4916980..4917396 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ51_RS23710	4912132..4913073	-	942	WP_001251335.1	ABC transporter permease	-
HHJ51_RS23715	4913074..4914087	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
HHJ51_RS23720	4914105..4915250	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
HHJ51_RS23725	4915495..4916901	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
HHJ51_RS23730	4916980..4917396	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HHJ51_RS23735	4917442..4917618	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HHJ51_RS23740	4917840..4918070	+	231	WP_000494244.1	YncJ family protein	-
HHJ51_RS23745	4918162..4920123	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HHJ51_RS23750	4920196..4920732	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
HHJ51_RS23755	4920785..4921996	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T156414 WP_000813797.1 NZ_CP053247:c4917618-4917442 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T156414 NZ_CP069064:2860109-2860522 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT156414 WP_076611057.1 NZ_CP053247:c4917396-4916980 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT156414 NZ_CP069064:2860561-2860818 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829J715