Detailed information of TA system TA156412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4817236..4818824
• Replicon: chromosome
• Accession: NZ_CP053247
• Organism: Escherichia coli strain SCU-482

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: HHJ51_RS23295
• Protein ID: WP_001125415.1
• Coordinates: 4817502..4818824 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HHJ51_RS23290
• Protein ID: WP_001296726.1
• Coordinates: 4817236..4817502 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ51_RS23290	4817236..4817502	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HHJ51_RS23295	4817502..4818824	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HHJ51_RS23300	4819222..4820121	-	900	WP_001057776.1	LysR family transcriptional regulator	-
HHJ51_RS23305	4820261..4820569	+	309	WP_001221046.1	hypothetical protein	-
HHJ51_RS23310	4820578..4821267	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
HHJ51_RS23315	4821341..4822288	+	948	WP_000854796.1	aldo/keto reductase	-
HHJ51_RS23320	4822391..4822546	+	156	WP_001322415.1	hypothetical protein	-
HHJ51_RS23325	4822506..4822925	+	420	WP_001322416.1	amidohydrolase family protein	-
HHJ51_RS23330	4822876..4823067	+	192	WP_001469139.1	amidohydrolase family protein	-
HHJ51_RS23335	4823218..4823790	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T156412 WP_001125415.1 NZ_CP053247:4817502-4818824 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T156412 NZ_CP069064:2799612-2800037 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT156412 WP_001296726.1 NZ_CP053247:4817236-4817502 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT156412 NZ_CP069064:2799385-2799606 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Toxin

• AlphaFold DB: U9YVT8