Detailed information of TA system TA156398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2429544..2430156
• Replicon: chromosome
• Accession: NZ_CP053247
• Organism: Escherichia coli strain SCU-482

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: HHJ51_RS11810
• Protein ID: WP_000833473.1
• Coordinates: 2429544..2429729 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: HHJ51_RS11815
• Protein ID: WP_000499744.1
• Coordinates: 2429746..2430156 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ51_RS11800	2426376..2427527	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
HHJ51_RS11805	2427728..2429266	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
HHJ51_RS11810	2429544..2429729	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
HHJ51_RS11815	2429746..2430156	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HHJ51_RS11820	2430228..2432192	-	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
HHJ51_RS11825	2432203..2433603	-	1401	WP_000204802.1	MFS transporter	-
HHJ51_RS11830	2433829..2434644	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T156398 WP_000833473.1 NZ_CP053247:2429544-2429729 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T156398 NZ_CP069064:1689951-1690268 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT156398 WP_000499744.1 NZ_CP053247:2429746-2430156 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT156398 NZ_CP069064:1689652-1689954 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCTGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9