Detailed information of TA system TA156385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4671353..4671943
• Replicon: chromosome
• Accession: NZ_CP053245
• Organism: Escherichia coli strain SCU-485

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V0VE57
• Locus tag: HHJ54_RS22365
• Protein ID: WP_000373724.1
• Coordinates: 4671353..4671685 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: HHJ54_RS22370
• Protein ID: WP_000288812.1
• Coordinates: 4671686..4671943 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ54_RS22340	4666816..4668078	+	1263	WP_000055690.1	integrase arm-type DNA-binding domain-containing protein	-
HHJ54_RS22345	4668217..4668675	-	459	WP_000526101.1	IS200/IS605 family transposase	-
HHJ54_RS22350	4669627..4670505	-	879	WP_000511773.1	integrase domain-containing protein	-
HHJ54_RS22355	4670643..4670969	+	327	Protein_4357	hypothetical protein	-
HHJ54_RS22360	4670950..4671306	+	357	WP_001147230.1	hypothetical protein	-
HHJ54_RS22365	4671353..4671685	-	333	WP_000373724.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HHJ54_RS22370	4671686..4671943	-	258	WP_000288812.1	antitoxin	Antitoxin
HHJ54_RS22375	4672041..4672232	+	192	WP_077561361.1	hypothetical protein	-
HHJ54_RS22380	4672291..4672497	-	207	WP_000983730.1	helix-turn-helix domain-containing protein	-
HHJ54_RS22385	4672494..4672961	-	468	WP_000201267.1	hypothetical protein	-
HHJ54_RS22390	4673194..4674330	-	1137	WP_001580729.1	ISAs1 family transposase	-
HHJ54_RS22395	4674483..4674632	+	150	Protein_4365	IS3 family transposase	-
HHJ54_RS22400	4674640..4675140	-	501	Protein_4366	type VI secretion system tip protein VgrG	-
HHJ54_RS22405	4675159..4675443	-	285	WP_001580451.1	type VI secretion system PAAR protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4656598..4675443	18845
	-	inside	IScluster/Tn	-	-	4668217..4674330	6113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11724.57 Da
• Isoelectric Point: 10.5834

>T156385 WP_000373724.1 NZ_CP053245:c4671685-4671353 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T156385 NZ_CP069064:714971-715378 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT156385 WP_000288812.1 NZ_CP053245:c4671943-4671686 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT156385 NZ_CP069064:714714-714974 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGTCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: V0VE57

Antitoxin

• AlphaFold DB: A0A3S7DDD0