Detailed information of TA system TA156376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4198615..4200203
• Replicon: chromosome
• Accession: NZ_CP053245
• Organism: Escherichia coli strain SCU-485

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HHJ54_RS19990
• Protein ID: WP_001125428.1
• Coordinates: 4198615..4199937 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: HHJ54_RS19995
• Protein ID: WP_001304368.1
• Coordinates: 4199937..4200203 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ54_RS19960	4193734..4194798	-	1065	WP_000859696.1	alpha/beta fold hydrolase	-
HHJ54_RS19965	4194956..4195717	-	762	WP_001066620.1	carboxymuconolactone decarboxylase family protein	-
HHJ54_RS19970	4195695..4196060	-	366	WP_000274389.1	cupin domain-containing protein	-
HHJ54_RS19975	4196562..4196909	-	348	WP_001337832.1	hypothetical protein	-
HHJ54_RS19980	4196911..4197405	-	495	WP_001337831.1	AEC family transporter	-
HHJ54_RS19985	4197948..4198361	+	414	WP_042115247.1	acetylxylan esterase	-
HHJ54_RS19990	4198615..4199937	-	1323	WP_001125428.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HHJ54_RS19995	4199937..4200203	-	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HHJ54_RS20000	4200511..4201269	+	759	WP_001286543.1	trans-aconitate 2-methyltransferase	-
HHJ54_RS20005	4201273..4202187	-	915	WP_001313828.1	bestrophin family inner membrane protein	-
HHJ54_RS20010	4202384..4203835	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	4178745..4200221	21476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.98 Da
• Isoelectric Point: 8.1297

>T156376 WP_001125428.1 NZ_CP053245:c4199937-4198615 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
FRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T156376 NZ_CP069064:359673-359975 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT156376 WP_001304368.1 NZ_CP053245:c4200203-4199937 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT156376 NZ_CP069064:360023-360244 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: V0VAH2