Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2658258..2658808 Replicon chromosome
Accession NZ_CP053245
Organism Escherichia coli strain SCU-485

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HHJ54_RS12400 Protein ID WP_001160966.1
Coordinates 2658494..2658808 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHJ54_RS12395 Protein ID WP_000125566.1
Coordinates 2658258..2658491 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ54_RS12370 2653331..2653618 + 288 WP_000203741.1 ferredoxin-like protein FixX -
HHJ54_RS12375 2653677..2655008 + 1332 WP_001183207.1 MFS transporter -
HHJ54_RS12380 2655116..2655646 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHJ54_RS12385 2655639..2657501 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
HHJ54_RS12390 2657693..2658172 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHJ54_RS12395 2658258..2658491 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHJ54_RS12400 2658494..2658808 + 315 WP_001160966.1 CcdB family protein Toxin
HHJ54_RS12405 2658805..2659653 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHJ54_RS12410 2659660..2660037 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHJ54_RS12415 2660040..2660861 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHJ54_RS12420 2660858..2661847 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHJ54_RS12425 2661847..2663133 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T156367 WP_001160966.1 NZ_CP053245:2658494-2658808 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T156367 NZ_CP069063:3172172-3172435 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT156367 WP_000125566.1 NZ_CP053245:2658258-2658491 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT156367 NZ_CP069063:3171887-3172168 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References