Detailed information of TA system TA156350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 60113..60738
• Replicon: plasmid pSCU-106-2
• Accession: NZ_CP053236
• Organism: Escherichia coli strain SCU-106

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HHJ24_RS26425
• Protein ID: WP_000911333.1
• Coordinates: 60340..60738 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HHJ24_RS26420
• Protein ID: WP_000450520.1
• Coordinates: 60113..60340 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ24_RS26420	60113..60340	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HHJ24_RS26425	60340..60738	+	399	WP_000911333.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HHJ24_RS26430	60747..62900	-	2154	WP_032330953.1	type IV conjugative transfer system coupling protein TraD	-
HHJ24_RS26435	63153..63884	-	732	WP_000850416.1	complement resistance protein TraT	-
HHJ24_RS26440	63898..64407	-	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id / sitABCD	iutA / iucD / iucC / iucB / iucA	1..111501	111501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14919.19 Da
• Isoelectric Point: 7.8605

>T156350 WP_000911333.1 NZ_CP053236:60340-60738 [Escherichia coli]
MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T156350 NZ_CP069063:c2240918-2240358 [Mycobacterium tuberculosis]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT156350 WP_000450520.1 NZ_CP053236:60113-60340 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT156350 NZ_CP069063:c2241256-2240915 [Mycobacterium tuberculosis]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2