Detailed information of TA system TA156344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4147180..4147805
• Replicon: chromosome
• Accession: NZ_CP053234
• Organism: Escherichia coli strain SCU-106

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HHJ24_RS20150
• Protein ID: WP_000911330.1
• Coordinates: 4147407..4147805 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HHJ24_RS20145
• Protein ID: WP_000450524.1
• Coordinates: 4147180..4147407 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ24_RS20120	4142983..4143453	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HHJ24_RS20125	4143453..4144025	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HHJ24_RS20130	4144171..4145049	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HHJ24_RS20135	4145066..4146100	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
HHJ24_RS20140	4146313..4147026	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HHJ24_RS20145	4147180..4147407	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HHJ24_RS20150	4147407..4147805	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HHJ24_RS20155	4147952..4148815	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
HHJ24_RS20160	4148830..4150845	+	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
HHJ24_RS20165	4150919..4151617	+	699	WP_000679812.1	esterase	-
HHJ24_RS20170	4151698..4151898	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T156344 WP_000911330.1 NZ_CP053234:4147407-4147805 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T156344 NZ_CP069063:c1944375-1943986 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT156344 WP_000450524.1 NZ_CP053234:4147180-4147407 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT156344 NZ_CP069063:c1944599-1944372 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33