Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3828563..3829146 Replicon chromosome
Accession NZ_CP053234
Organism Escherichia coli strain SCU-106

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag HHJ24_RS18535 Protein ID WP_000254745.1
Coordinates 3828811..3829146 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2J1DJJ7
Locus tag HHJ24_RS18530 Protein ID WP_000581939.1
Coordinates 3828563..3828811 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ24_RS18520 3824902..3826203 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HHJ24_RS18525 3826251..3828485 + 2235 WP_170969484.1 GTP diphosphokinase -
HHJ24_RS18530 3828563..3828811 + 249 WP_000581939.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HHJ24_RS18535 3828811..3829146 + 336 WP_000254745.1 endoribonuclease MazF Toxin
HHJ24_RS18540 3829217..3830008 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HHJ24_RS18545 3830236..3831873 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HHJ24_RS18550 3831961..3833259 + 1299 WP_000036723.1 phosphopyruvate hydratase -
HHJ24_RS18555 3833315..3833677 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

Antitoxin PemI-like

(2-82)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T156343 WP_000254745.1 NZ_CP053234:3828811-3829146 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T156343 NZ_CP069063:1771097-1771501 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4791

>AT156343 WP_000581939.1 NZ_CP053234:3828563-3828811 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT156343 NZ_CP069063:1770888-1771091 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DJJ7

References