Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3653409..3654063 Replicon chromosome
Accession NZ_CP053234
Organism Escherichia coli strain SCU-106

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HHJ24_RS17765 Protein ID WP_000244772.1
Coordinates 3653656..3654063 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ24_RS17760 Protein ID WP_000354046.1
Coordinates 3653409..3653675 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ24_RS17735 3648578..3649321 + 744 WP_000951964.1 SDR family oxidoreductase -
HHJ24_RS17740 3649378..3650811 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
HHJ24_RS17745 3650856..3651167 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HHJ24_RS17750 3651331..3651990 + 660 WP_000250274.1 hemolysin III family protein -
HHJ24_RS17755 3652186..3653166 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HHJ24_RS17760 3653409..3653675 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ24_RS17765 3653656..3654063 + 408 WP_000244772.1 protein YgfX Toxin
HHJ24_RS17770 3654103..3654624 - 522 WP_001055874.1 flavodoxin FldB -
HHJ24_RS17775 3654736..3655632 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ24_RS17780 3655657..3656367 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ24_RS17785 3656373..3658106 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T156342 WP_000244772.1 NZ_CP053234:3653656-3654063 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156342 NZ_CP069063:1690531-1690848 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156342 WP_000354046.1 NZ_CP053234:3653409-3653675 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156342 NZ_CP069063:1690232-1690534 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References