Detailed information of TA system TA156336

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2228005..2228837
• Replicon: chromosome
• Accession: NZ_CP053234
• Organism: Escherichia coli strain SCU-106

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HHJ24_RS10980
• Protein ID: WP_000854816.1
• Coordinates: 2228463..2228837 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ24_RS10975
• Protein ID: WP_001285402.1
• Coordinates: 2228005..2228373 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ24_RS10945	2224646..2225101	+	456	WP_024226900.1	hypothetical protein	-
HHJ24_RS10950	2225176..2225409	+	234	Protein_2143	DUF905 family protein	-
HHJ24_RS10955	2225500..2226318	+	819	WP_001234638.1	DUF945 domain-containing protein	-
HHJ24_RS10960	2226581..2227060	+	480	WP_000706981.1	antirestriction protein	-
HHJ24_RS10965	2227076..2227552	+	477	WP_001186727.1	RadC family protein	-
HHJ24_RS10970	2227621..2227842	+	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ24_RS10975	2228005..2228373	+	369	WP_001285402.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ24_RS10980	2228463..2228837	+	375	WP_000854816.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ24_RS10985	2228834..2229325	+	492	WP_000976864.1	hypothetical protein	-
HHJ24_RS10990	2229337..2229534	+	198	WP_032209866.1	DUF957 domain-containing protein	-
HHJ24_RS10995	2229619..2230170	+	552	WP_001274563.1	DUF4942 domain-containing protein	-
HHJ24_RS11000	2230252..2230677	+	426	WP_000422722.1	IS66 family insertion sequence hypothetical protein	-
HHJ24_RS11005	2230674..2231024	+	351	WP_000624684.1	IS66 family insertion sequence element accessory protein TnpB	-
HHJ24_RS11010	2231055..2232668	+	1614	WP_170969401.1	IS66 family transposase	-
HHJ24_RS11015	2232687..2233001	+	315	Protein_2156	DUF4942 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14045.05 Da
• Isoelectric Point: 9.1493

>T156336 WP_000854816.1 NZ_CP053234:2228463-2228837 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAK

Nucleotide

• Length: 375 bp

>T156336 NZ_CP069063:c1231504-1231193 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13883.62 Da
• Isoelectric Point: 6.3165

>AT156336 WP_001285402.1 NZ_CP053234:2228005-2228373 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT156336 NZ_CP069063:c1231824-1231504 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A