156325

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 188461..189298 Replicon chromosome
Accession NZ_CP053234
Organism Escherichia coli strain SCU-106

Toxin (Protein)


Gene name itaT Uniprot ID A0A5E8NZI9
Locus tag HHJ24_RS00885 Protein ID WP_001503903.1
Coordinates 188461..189003 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HHJ24_RS00890 Protein ID WP_001297137.1
Coordinates 188987..189298 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ24_RS00865 184000..184911 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
HHJ24_RS00870 185079..185570 + 492 WP_001138904.1 nucleotide binding protein YajQ -
HHJ24_RS00875 185698..187062 - 1365 WP_170969251.1 MFS transporter -
HHJ24_RS00880 187470..188405 + 936 WP_001297127.1 sel1 repeat family protein -
HHJ24_RS00885 188461..189003 - 543 WP_001503903.1 GNAT family N-acetyltransferase Toxin
HHJ24_RS00890 188987..189298 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HHJ24_RS00895 189483..190373 - 891 WP_000971336.1 heme o synthase -
HHJ24_RS00900 190385..190714 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HHJ24_RS00905 190714..191328 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HHJ24_RS00910 191318..193309 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HHJ24_RS00915 193331..194278 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19835.05 Da        Isoelectric Point: 8.3395

>T156325 WP_001503903.1 NZ_CP053234:c189003-188461 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAT

Download         Length: 543 bp

>T156325 NZ_CP069063:706444-706845 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT156325 WP_001297137.1 NZ_CP053234:c189298-188987 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT156325 NZ_CP069063:706202-706447 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E8NZI9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References