156314

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 15080..15687 Replicon plasmid pSCU-306-1
Accession NZ_CP053232
Organism Escherichia coli strain SCU-306

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag HHJ44_RS24310 Protein ID WP_000673985.1
Coordinates 15301..15687 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag HHJ44_RS24305 Protein ID WP_000493532.1
Coordinates 15080..15304 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ44_RS24285 11172..11589 - 418 Protein_12 polymer-forming cytoskeletal protein -
HHJ44_RS24290 12479..12733 - 255 Protein_13 hypothetical protein -
HHJ44_RS24295 12826..13998 + 1173 Protein_14 IS21-like element IS21 family transposase -
HHJ44_RS24300 13998..14795 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
HHJ44_RS24305 15080..15304 + 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HHJ44_RS24310 15301..15687 + 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HHJ44_RS24315 16465..18171 - 1707 WP_170969095.1 ABC transporter ATP-binding protein/permease -
HHJ44_RS24320 18188..18868 - 681 Protein_19 lasso peptide biosynthesis B2 protein -
HHJ44_RS24325 18868..20254 - 1387 Protein_20 hypothetical protein -
HHJ44_RS24330 20382..20569 - 188 Protein_21 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / sul1 / qacE / aadA2 / dfrA12 senB 1..128584 128584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T156314 WP_000673985.1 NZ_CP053232:15301-15687 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T156314 NZ_CP069062:c3805129-3804737 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT156314 WP_000493532.1 NZ_CP053232:15080-15304 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT156314 NZ_CP069062:c3805404-3805129 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References