Detailed information of TA system TA156310

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4240043..4240401
• Replicon: chromosome
• Accession: NZ_CP053231
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HHJ44_RS20510
• Protein ID: WP_001332330.1
• Coordinates: 4240279..4240401 (+)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: HHJ44_RS20505
• Protein ID: WP_000125566.1
• Coordinates: 4240043..4240276 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS20480	4235116..4235403	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
HHJ44_RS20485	4235462..4236793	+	1332	WP_001183207.1	MFS transporter	-
HHJ44_RS20490	4236901..4237431	+	531	WP_000600749.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
HHJ44_RS20495	4237424..4239286	+	1863	WP_000377122.1	glutathione-regulated potassium-efflux system protein KefC	-
HHJ44_RS20500	4239478..4239957	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
HHJ44_RS20505	4240043..4240276	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HHJ44_RS20510	4240279..4240401	+	123	WP_001332330.1	CcdB family protein	Toxin
HHJ44_RS20515	4240442..4240594	+	153	WP_146692624.1	CcdB family protein	-
HHJ44_RS20520	4240591..4241439	-	849	WP_000257201.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
HHJ44_RS20525	4241446..4241823	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
HHJ44_RS20530	4241826..4242647	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
HHJ44_RS20535	4242644..4243633	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
HHJ44_RS20540	4243633..4244919	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-39)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4563.34 Da
• Isoelectric Point: 10.9266

>T156310 WP_001332330.1 NZ_CP053231:4240279-4240401 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPI

Nucleotide

• Length: 123 bp

>T156310 NZ_CP069062:3184860-3185303 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT156310 WP_000125566.1 NZ_CP053231:4240043-4240276 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT156310 NZ_CP069062:3184616-3184873 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9