Detailed information of TA system TA156305

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4019993..4020827
• Replicon: chromosome
• Accession: NZ_CP053231
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: HHJ44_RS19380
• Protein ID: WP_000854770.1
• Coordinates: 4020450..4020827 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ44_RS19375
• Protein ID: WP_001332042.1
• Coordinates: 4019993..4020361 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS19345	4017117..4017275	+	159	WP_001323397.1	DUF905 family protein	-
HHJ44_RS19350	4017430..4018248	+	819	WP_001234738.1	DUF945 domain-containing protein	-
HHJ44_RS19355	4018248..4018495	+	248	Protein_3783	antirestriction protein	-
HHJ44_RS19360	4018590..4019054	+	465	WP_000855061.1	antirestriction protein	-
HHJ44_RS19365	4019070..4019546	+	477	WP_001557203.1	RadC family protein	-
HHJ44_RS19370	4019609..4019830	+	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ44_RS19375	4019993..4020361	+	369	WP_001332042.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ44_RS19380	4020450..4020827	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
HHJ44_RS19385	4020824..4021312	+	489	WP_000761690.1	hypothetical protein	-
HHJ44_RS19390	4021329..4021505	+	177	WP_000839286.1	DUF957 domain-containing protein	-
HHJ44_RS19395	4021423..4021613	+	191	Protein_3791	hypothetical protein	-
HHJ44_RS19400	4022127..4022303	+	177	Protein_3792	helix-turn-helix domain-containing protein	-
HHJ44_RS19405	4022785..4024322	+	1538	WP_085953974.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T156305 WP_000854770.1 NZ_CP053231:4020450-4020827 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T156305 NZ_CP069062:c3068746-3068351 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13677.38 Da
• Isoelectric Point: 6.5931

>AT156305 WP_001332042.1 NZ_CP053231:4019993-4020361 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT156305 NZ_CP069062:c3068325-3068059 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9