Detailed information of TA system TA156304

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3763689..3764523
• Replicon: chromosome
• Accession: NZ_CP053231
• Organism: Escherichia coli strain SCU-306

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: HHJ44_RS18075
• Protein ID: WP_000854820.1
• Coordinates: 3763689..3764066 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: HHJ44_RS18080
• Protein ID: WP_001285610.1
• Coordinates: 3764155..3764523 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ44_RS18055	3760252..3761790	-	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-
HHJ44_RS18060	3762622..3763185	-	564	WP_001280448.1	DUF4942 domain-containing protein	-
HHJ44_RS18065	3763270..3763467	-	198	WP_085949158.1	DUF957 domain-containing protein	-
HHJ44_RS18070	3763495..3763692	-	198	Protein_3531	hypothetical protein	-
HHJ44_RS18075	3763689..3764066	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ44_RS18080	3764155..3764523	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ44_RS18085	3764603..3764824	-	222	WP_000692347.1	DUF987 domain-containing protein	-
HHJ44_RS18090	3764911..3765387	-	477	WP_170969066.1	RadC family protein	-
HHJ44_RS18095	3765402..3765856	-	455	Protein_3536	antirestriction protein	-
HHJ44_RS18100	3766146..3766962	-	817	Protein_3537	DUF945 domain-containing protein	-
HHJ44_RS18105	3767052..3767284	-	233	Protein_3538	DUF905 family protein	-
HHJ44_RS18110	3767290..3767576	-	287	Protein_3539	hypothetical protein	-
HHJ44_RS18115	3767639..3767965	-	327	WP_170969067.1	hypothetical protein	-
HHJ44_RS18120	3768116..3768795	-	680	Protein_3541	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T156304 WP_000854820.1 NZ_CP053231:c3764066-3763689 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T156304 NZ_CP069062:c3068062-3067646 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT156304 WP_001285610.1 NZ_CP053231:c3764523-3764155 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT156304 NZ_CP069062:c3068325-3068059 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6