Detailed information of TA system TA156286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 20410..20956
• Replicon: plasmid pCbo51
• Accession: NZ_CP053230
• Organism: Enterocloster bolteae strain CBBP-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R0AEL6
• Locus tag: HLY09_RS30150
• Protein ID: WP_002571125.1
• Coordinates: 20410..20688 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HLY09_RS30155
• Protein ID: WP_171332586.1
• Coordinates: 20681..20956 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY09_RS30125	15552..15926	+	375	WP_171332605.1	BlaI/MecI/CopY family transcriptional regulator	-
HLY09_RS30130	15916..17316	+	1401	WP_171332582.1	M56 family metallopeptidase	-
HLY09_RS30135	17926..18633	+	708	WP_171332583.1	hypothetical protein	-
HLY09_RS30140	18812..19408	+	597	WP_171332584.1	hypothetical protein	-
HLY09_RS30145	19459..19920	+	462	WP_171332585.1	hypothetical protein	-
HLY09_RS30150	20410..20688	-	279	WP_002571125.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HLY09_RS30155	20681..20956	-	276	WP_171332586.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HLY09_RS30160	21582..21794	+	213	WP_171332587.1	hypothetical protein	-
HLY09_RS30165	21884..22627	+	744	WP_039897592.1	AAA family ATPase	-
HLY09_RS30170	23186..23443	+	258	WP_002571130.1	hypothetical protein	-
HLY09_RS30175	23567..24364	+	798	WP_171332588.1	hypothetical protein	-
HLY09_RS30180	24354..25813	+	1460	Protein_26	tyrosine-type recombinase/integrase	-
HLY09_RS30185	25794..25943	+	150	WP_171332589.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51398	51398

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-89)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10962.65 Da
• Isoelectric Point: 8.4762

>T156286 WP_002571125.1 NZ_CP053230:c20688-20410 [Enterocloster bolteae]
MLKIEYQGQFKRDYKLALKRGCNPELLTEVISILANEQELPEKYRDHSLTNSRNYKNVRECHIQPDWLLIYQINKDILTL
KLLRTGSHSDLF

Nucleotide

• Length: 279 bp

>T156286 NZ_CP069062:c1942693-1942304 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGCGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10309.72 Da
• Isoelectric Point: 4.2813

>AT156286 WP_171332586.1 NZ_CP053230:c20956-20681 [Enterocloster bolteae]
MSEKNTSMNIRMNKDVKLQAQKIFNDLGIDLTTAVNVFLLQSIRYQGFPFDVTLRQEPNTTTMAAIENADKEIDMHGPFE
SVEALMEDLNA

Nucleotide

• Length: 276 bp

>AT156286 NZ_CP069062:c1942917-1942690 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A6M5GDM8