Detailed information of TA system TA156285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 56431..56962
• Replicon: plasmid pRSMS1
• Accession: NZ_CP053224
• Organism: Sphingobium sp. RSMS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T0GNF5
• Locus tag: EGM87_RS23410
• Protein ID: WP_004212690.1
• Coordinates: 56681..56962 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: T0G968
• Locus tag: EGM87_RS23405
• Protein ID: WP_004212689.1
• Coordinates: 56431..56694 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM87_RS23385	51606..53312	+	1707	WP_062343367.1	copper resistance system multicopper oxidase	-
EGM87_RS23390	53309..54295	+	987	WP_004212678.1	copper resistance protein B	-
EGM87_RS23395	54289..55530	+	1242	WP_007683345.1	MFS transporter	-
EGM87_RS23400	55610..56047	+	438	WP_007683347.1	metal-binding protein	-
EGM87_RS23405	56431..56694	+	264	WP_004212689.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EGM87_RS23410	56681..56962	+	282	WP_004212690.1	type II toxin-antitoxin system YafQ family toxin	Toxin
EGM87_RS23415	57364..58056	+	693	Protein_55	IS21 family transposase	-
EGM87_RS23420	58043..58771	+	729	WP_025549137.1	IS21-like element helper ATPase IstB	-
EGM87_RS23425	58973..59401	+	429	WP_021245211.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152994	152994
	-	flank	IS/Tn	-	-	58043..58771	728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10993.49 Da
• Isoelectric Point: 7.4752

>T156285 WP_004212690.1 NZ_CP053224:56681-56962 [Sphingobium sp. RSMS]
MRTIERFGRFKRDYKREKKGQHAKTLDADLIPIIEALASDEPLEPRHRDHALTGDWRDHRDCHIKPDLVLIYRKPDDDTL
QLVRLGSHAELGW

Nucleotide

• Length: 282 bp

>T156285 NZ_CP069062:1769358-1769762 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9284.75 Da
• Isoelectric Point: 4.5881

>AT156285 WP_004212689.1 NZ_CP053224:56431-56694 [Sphingobium sp. RSMS]
MAANALVQTRIDGAVKEEAATVLAAMGLTVSDAVRLMLTRVARDKALPFEPLVPNAETIEAMKEAREGKGKRFATVVDLM
ADLNADD

Nucleotide

• Length: 264 bp

>AT156285 NZ_CP069062:1769149-1769352 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: T0GNF5

Antitoxin

• AlphaFold DB: T0G968