Detailed information of TA system TA156284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 222529..223103
• Replicon: chromosome
• Accession: NZ_CP053223
• Organism: Sphingobium sp. RSMS

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EGM87_RS19090
• Protein ID: WP_137709072.1
• Coordinates: 222771..223103 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EGM87_RS19085
• Protein ID: WP_082166021.1
• Coordinates: 222529..222771 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM87_RS19045	217644..218422	-	779	Protein_186	SDR family oxidoreductase	-
EGM87_RS19050	218484..218813	-	330	WP_048575606.1	nuclear transport factor 2 family protein	-
EGM87_RS19055	218954..219874	+	921	WP_137709073.1	LysR family transcriptional regulator	-
EGM87_RS19060	219948..220322	+	375	WP_044660862.1	hypothetical protein	-
EGM87_RS19065	220569..220781	-	213	WP_099186724.1	hypothetical protein	-
EGM87_RS19070	220898..221161	+	264	Protein_191	transposase	-
EGM87_RS19075	221250..221564	-	315	WP_048575603.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EGM87_RS19080	221570..221827	-	258	WP_048575602.1	addiction module antitoxin	-
EGM87_RS19085	222529..222771	+	243	WP_082166021.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EGM87_RS19090	222771..223103	+	333	WP_137709072.1	endoribonuclease MazF	Toxin
EGM87_RS19095	223378..223647	+	270	WP_044660855.1	type II toxin-antitoxin system ParD family antitoxin	-
EGM87_RS19100	223647..224093	+	447	WP_082166019.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EGM87_RS19105	224062..224199	+	138	WP_158230081.1	hypothetical protein	-
EGM87_RS19110	224316..224831	-	516	WP_103001489.1	hypothetical protein	-
EGM87_RS19115	225202..225354	+	153	Protein_200	DUF736 domain-containing protein	-
EGM87_RS19120	225345..226448	+	1104	WP_137709071.1	TonB-dependent receptor	-
EGM87_RS19125	226562..226705	+	144	WP_157009786.1	hypothetical protein	-
EGM87_RS19130	226764..227084	+	321	WP_137709070.1	hypothetical protein	-
EGM87_RS19135	227153..227815	+	663	Protein_204	toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	220886..221200	314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-109)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12114.34 Da
• Isoelectric Point: 9.9037

>T156284 WP_137709072.1 NZ_CP053223:222771-223103 [Sphingobium sp. RSMS]
MAKYVPDMGDIVWLNFDPQAGHEQAGHRPALVLSPAAYNRMRGMMICCPMTSKIKGYPFEVLISQNLPSAVLADQLKSVD
WMTRKATKKGTVSQAALLEVQAKIRALLAI

Nucleotide

• Length: 333 bp

>T156284 NZ_CP069062:1691093-1691410 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8799.10 Da
• Isoelectric Point: 4.5489

>AT156284 WP_082166021.1 NZ_CP053223:222529-222771 [Sphingobium sp. RSMS]
MRVRVKKWGNSASVRIPSSIMAAAALHIDQEVDVREEEGRVIIEPITAPAFTLEGLLAGMKPENFPENVDFGPPVGNELW

Nucleotide

• Length: 243 bp

>AT156284 NZ_CP069062:1690794-1691096 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA