Detailed information of TA system TA156273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 31259..31881
• Replicon: plasmid unnamed1
• Accession: NZ_CP053216
• Organism: Acinetobacter baumannii strain DT0544C

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HLG75_RS19100
• Protein ID: WP_140948450.1
• Coordinates: 31486..31881 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HLG75_RS19095
• Protein ID: WP_104427085.1
• Coordinates: 31259..31489 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG75_RS19080	28920..29486	+	567	WP_121775131.1	recombinase family protein	-
HLG75_RS19085	29740..30171	-	432	WP_000042163.1	MarR family transcriptional regulator	-
HLG75_RS19090	30178..30606	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
HLG75_RS19095	31259..31489	+	231	WP_104427085.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HLG75_RS19100	31486..31881	+	396	WP_140948450.1	type II toxin-antitoxin system VapC family toxin	Toxin
HLG75_RS19105	31961..32563	+	603	WP_140948449.1	glutamine ABC transporter permease GlnP	-
HLG75_RS19110	32611..32790	+	180	WP_000902128.1	hypothetical protein	-
HLG75_RS19115	32944..33759	-	816	WP_000018326.1	aminoglycoside O-phosphotransferase APH(3')-Ia	-
HLG75_RS19120	33810..34343	-	534	WP_000381802.1	aminoglycoside nucleotidyltransferase ANT(2'')-Ia	-
HLG75_RS19125	34480..35184	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HLG75_RS19130	35236..35640	-	405	Protein_45	TrbG/VirB9 family P-type conjugative transfer protein	-
HLG75_RS19135	35652..36437	-	786	Protein_46	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / dfrA1 / ant(3'')-Ia / blaCARB-5 / aph(3')-Ia / ant(2'')-Ia / cmlB1	-	1..55394	55394
	-	flank	IS/Tn	aph(3')-Ia / ant(2'')-Ia	-	32944..35184	2240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14925.20 Da
• Isoelectric Point: 6.2238

>T156273 WP_140948450.1 NZ_CP053216:31486-31881 [Acinetobacter baumannii]
MIYLLDTNICIYVINHKPQQVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKQALNKFLSPLDILPYDEQAIWHYA
KLRQDLQSTGKTIGSLDMLIAAHALALDVVLVTNNIKEFERIDGLKLENWT

Nucleotide

• Length: 396 bp

>T156273 NZ_CP069062:c759174-758737 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8733.00 Da
• Isoelectric Point: 4.7708

>AT156273 WP_104427085.1 NZ_CP053216:31259-31489 [Acinetobacter baumannii]
METAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIENPWDIMLEAINEFEVGFQLERADQGEQLREDFK

Nucleotide

• Length: 231 bp

>AT156273 NZ_CP069062:c758627-758382 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA