Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3293070..3293688 Replicon chromosome
Accession NZ_CP053211
Organism Escherichia coli strain QDFD216

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HJ589_RS15965 Protein ID WP_001291435.1
Coordinates 3293470..3293688 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HJ589_RS15960 Protein ID WP_000344800.1
Coordinates 3293070..3293444 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJ589_RS15950 (3288159) 3288159..3289352 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HJ589_RS15955 (3289375) 3289375..3292524 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HJ589_RS15960 (3293070) 3293070..3293444 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HJ589_RS15965 (3293470) 3293470..3293688 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HJ589_RS15970 (3293860) 3293860..3294411 + 552 WP_000102564.1 maltose O-acetyltransferase -
HJ589_RS15975 (3294527) 3294527..3294997 + 471 WP_000136192.1 YlaC family protein -
HJ589_RS15980 (3295161) 3295161..3296709 + 1549 Protein_3161 cyclic-guanylate-specific phosphodiesterase PdeB -
HJ589_RS15985 (3296751) 3296751..3297104 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HJ589_RS15995 (3297483) 3297483..3297794 + 312 WP_000409911.1 MGMT family protein -
HJ589_RS16000 (3297825) 3297825..3298397 - 573 WP_077477690.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T156263 WP_001291435.1 NZ_CP053211:3293470-3293688 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T156263 NZ_CP069062:c643484-643071 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT156263 WP_000344800.1 NZ_CP053211:3293070-3293444 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT156263 NZ_CP069062:c643747-643481 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References