Detailed information of TA system TA156244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 409624..410204
• Replicon: plasmid pRltTA1A
• Accession: NZ_CP053209
• Organism: Rhizobium leguminosarum bv. trifolii TA1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RLTA1_RS36230
• Protein ID: WP_027688371.1
• Coordinates: 409821..410204 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RLTA1_RS36225
• Protein ID: WP_027688372.1
• Coordinates: 409624..409824 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLTA1_RS36195	404799..405557	-	759	WP_027688378.1	ImuA family protein	-
RLTA1_RS36200	405732..405995	+	264	WP_027688377.1	hypothetical protein	-
RLTA1_RS36205	405999..407074	+	1076	Protein_413	DNA polymerase IV	-
RLTA1_RS36210	407324..407809	-	486	WP_027688376.1	hypothetical protein	-
RLTA1_RS36215	408060..408317	+	258	WP_027688375.1	hypothetical protein	-
RLTA1_RS36220	408715..409158	-	444	WP_027688374.1	GNAT family N-acetyltransferase	-
RLTA1_RS36225	409624..409824	+	201	WP_027688372.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
RLTA1_RS36230	409821..410204	+	384	WP_027688371.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLTA1_RS36235	410504..411394	-	891	WP_027688370.1	LysR family transcriptional regulator	-
RLTA1_RS36240	411492..411827	+	336	WP_085065853.1	helix-turn-helix transcriptional regulator	-
RLTA1_RS36245	412085..412651	-	567	WP_027688369.1	hypothetical protein	-
RLTA1_RS36250	412648..413877	-	1230	WP_027688368.1	AAA family ATPase	-
RLTA1_RS36255	413950..414108	+	159	WP_156925142.1	hypothetical protein	-
RLTA1_RS36260	414105..414362	-	258	WP_130663815.1	hypothetical protein	-
RLTA1_RS36265	414359..414860	-	502	Protein_425	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..498418	498418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14229.45 Da
• Isoelectric Point: 8.1753

>T156244 WP_027688371.1 NZ_CP053209:409821-410204 [Rhizobium leguminosarum bv. trifolii TA1]
VILADTSIWSDHFRHMNAELRWVIEGDRLLCHPSVIGELALGSLRDRRSVLAFLTAQRKAIVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASVLLEQRAALWTRDKRLRAAAEKAGAALHIPVDRSH

Nucleotide

• Length: 384 bp

>T156244 NZ_CP069046:c2903560-2902238 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7110.18 Da
• Isoelectric Point: 11.1107

>AT156244 WP_027688372.1 NZ_CP053209:409624-409824 [Rhizobium leguminosarum bv. trifolii TA1]
MRSTINLDDTLLDKARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSTASK

Nucleotide

• Length: 201 bp

>AT156244 NZ_CP069046:c2903826-2903560 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA