Detailed information of TA system TA156242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 654825..655360
• Replicon: plasmid pRltTA1D
• Accession: NZ_CP053206
• Organism: Rhizobium leguminosarum bv. trifolii TA1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RLTA1_RS27690
• Protein ID: WP_027688291.1
• Coordinates: 655085..655360 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8I2GF38
• Locus tag: RLTA1_RS27685
• Protein ID: WP_027688292.1
• Coordinates: 654825..655085 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLTA1_RS27665	650620..651501	-	882	WP_003549874.1	ABC transporter permease	-
RLTA1_RS27670	651513..652331	-	819	WP_026170969.1	ABC transporter ATP-binding protein	-
RLTA1_RS27675	652426..653439	-	1014	WP_018072979.1	ABC transporter substrate-binding protein	-
RLTA1_RS27680	653793..654521	-	729	WP_024319649.1	FadR family transcriptional regulator	-
RLTA1_RS27685	654825..655085	+	261	WP_027688292.1	ribbon-helix-helix domain-containing protein	Antitoxin
RLTA1_RS27690	655085..655360	+	276	WP_027688291.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RLTA1_RS27695	655367..656374	-	1008	WP_024319652.1	LacI family transcriptional regulator	-
RLTA1_RS27700	656582..657904	+	1323	WP_027688290.1	alpha-L-fucosidase	-
RLTA1_RS27705	657955..659178	+	1224	WP_018072986.1	sugar ABC transporter substrate-binding protein	-
RLTA1_RS27710	659261..660235	+	975	WP_018482413.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..805040	805040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10814.49 Da
• Isoelectric Point: 10.2922

>T156242 WP_027688291.1 NZ_CP053206:655085-655360 [Rhizobium leguminosarum bv. trifolii TA1]
MGIKWTSKAASDVARLYDFLTPVDRQAAVRTVRALTAAPARLLEQPRLGERLEEFDPREVRRILVGRYELRYEIQQSMIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T156242 NZ_CP069046:883679-884089 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9756.97 Da
• Isoelectric Point: 4.4964

>AT156242 WP_027688292.1 NZ_CP053206:654825-655085 [Rhizobium leguminosarum bv. trifolii TA1]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDDGNLVEHQSVQAWADSLDSDE
PISLPR

Nucleotide

• Length: 261 bp

>AT156242 NZ_CP069046:883432-883698 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA