Detailed information of TA system TA156241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 270497..271173
• Replicon: plasmid pRltTA1D
• Accession: NZ_CP053206
• Organism: Rhizobium leguminosarum bv. trifolii TA1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RLTA1_RS25930
• Protein ID: WP_027688084.1
• Coordinates: 270748..271173 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4Q8ZXM1
• Locus tag: RLTA1_RS25925
• Protein ID: WP_075224380.1
• Coordinates: 270497..270751 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLTA1_RS25895	265851..266744	-	894	WP_027688088.1	NmrA family NAD(P)-binding protein	-
RLTA1_RS25900	266812..267237	-	426	WP_027688087.1	MarR family winged helix-turn-helix transcriptional regulator	-
RLTA1_RS25905	267436..267839	+	404	Protein_248	hypothetical protein	-
RLTA1_RS25910	267913..268878	-	966	WP_027688086.1	alpha/beta hydrolase	-
RLTA1_RS25915	268975..269661	-	687	WP_027688085.1	glutathione S-transferase	-
RLTA1_RS25920	269766..270341	+	576	WP_018480381.1	TetR/AcrR family transcriptional regulator	-
RLTA1_RS25925	270497..270751	+	255	WP_075224380.1	plasmid stabilization protein	Antitoxin
RLTA1_RS25930	270748..271173	+	426	WP_027688084.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLTA1_RS25935	271619..272407	+	789	WP_027688083.1	siderophore-iron reductase FhuF	-
RLTA1_RS25940	272503..272685	-	183	WP_042940897.1	hypothetical protein	-
RLTA1_RS25945	273590..275467	+	1878	WP_024321535.1	methyl-accepting chemotaxis protein	-
RLTA1_RS25950	275664..276053	+	390	WP_027688081.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..805040	805040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15186.42 Da
• Isoelectric Point: 4.8283

>T156241 WP_027688084.1 NZ_CP053206:270748-271173 [Rhizobium leguminosarum bv. trifolii TA1]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLDDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARAAGRAIGKADAYIAATAAANGFIVATRDSSPFEAAGLKVINPWYAPT

Nucleotide

• Length: 426 bp

>T156241 NZ_CP069046:750616-751101 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9249.43 Da
• Isoelectric Point: 5.6158

>AT156241 WP_075224380.1 NZ_CP053206:270497-270751 [Rhizobium leguminosarum bv. trifolii TA1]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNDDVDNLQLVRDKTPAEP
MSFE

Nucleotide

• Length: 255 bp

>AT156241 NZ_CP069046:750326-750619 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4Q8ZXM1