Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 60974..61665 Replicon plasmid pRltTA1D
Accession NZ_CP053206
Organism Rhizobium leguminosarum bv. trifolii TA1

Toxin (Protein)


Gene name vapC Uniprot ID A0A4Q8ZX68
Locus tag RLTA1_RS24955 Protein ID WP_027688184.1
Coordinates 60974..61402 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A4R0AD13
Locus tag RLTA1_RS24960 Protein ID WP_017991284.1
Coordinates 61399..61665 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLTA1_RS24935 57869..58708 + 840 WP_027688185.1 transporter substrate-binding domain-containing protein -
RLTA1_RS24940 58826..59488 + 663 WP_003550217.1 amino acid ABC transporter permease -
RLTA1_RS24945 59495..60154 + 660 WP_024319797.1 amino acid ABC transporter permease -
RLTA1_RS24950 60167..60937 + 771 WP_018480586.1 amino acid ABC transporter ATP-binding protein -
RLTA1_RS24955 60974..61402 - 429 WP_027688184.1 type II toxin-antitoxin system VapC family toxin Toxin
RLTA1_RS24960 61399..61665 - 267 WP_017991284.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RLTA1_RS24965 61922..62665 + 744 WP_027688183.1 GntR family transcriptional regulator -
RLTA1_RS24970 62669..63454 + 786 WP_024319800.1 transporter substrate-binding domain-containing protein -
RLTA1_RS24975 63536..64207 + 672 WP_024319801.1 amino acid ABC transporter permease -
RLTA1_RS24980 64204..64863 + 660 WP_024319802.1 amino acid ABC transporter permease -
RLTA1_RS24985 64841..65566 + 726 WP_024319803.1 amino acid ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..805040 805040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-66)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15824.14 Da        Isoelectric Point: 4.7948

>T156240 WP_027688184.1 NZ_CP053206:c61402-60974 [Rhizobium leguminosarum bv. trifolii TA1]
VRLLLDTNVLSEVTRPRPDAHVLQWLDDLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QAVAITWGDLMGLAKRSGRGLSSMGGLIAATAIAHDLTLATRNIKDFESLGIELVDPWIERP

Download         Length: 429 bp

>T156240 NZ_CP069046:363240-363587 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9960.34 Da        Isoelectric Point: 10.0842

>AT156240 WP_017991284.1 NZ_CP053206:c61665-61399 [Rhizobium leguminosarum bv. trifolii TA1]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL

Download         Length: 267 bp

>AT156240 NZ_CP069046:363587-363886 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q8ZX68

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0AD13

References