Detailed information of TA system TA156225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1843205..1843811
• Replicon: chromosome
• Accession: NZ_CP053190
• Organism: Enterobacter hormaechei strain EGYMCRVIM

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: HK406_RS08935
• Protein ID: WP_012906750.1
• Coordinates: 1843632..1843811 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HK406_RS08930
• Protein ID: WP_044703865.1
• Coordinates: 1843205..1843609 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HK406_RS08910	1838709..1839260	+	552	WP_044703861.1	phage tail protein I	-
HK406_RS08915	1839263..1841440	+	2178	WP_170851313.1	phage tail protein	-
HK406_RS08920	1841440..1842018	+	579	WP_032619505.1	tail fiber assembly protein	-
HK406_RS08925	1842095..1843165	+	1071	WP_044703863.1	glycosyltransferase family 9 protein	-
HK406_RS08930	1843205..1843609	-	405	WP_044703865.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HK406_RS08935	1843632..1843811	-	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
HK406_RS08940	1844097..1844249	+	153	WP_023292711.1	isocitrate dehydrogenase (NADP(+))	-
HK406_RS08945	1844325..1844570	+	246	WP_022650806.1	YmjA family protein	-
HK406_RS08950	1844575..1846074	-	1500	WP_047055268.1	EAL domain-containing protein	-
HK406_RS08955	1846199..1846291	-	93	WP_071524166.1	stress response membrane protein YncL	-
HK406_RS08960	1846662..1846910	+	249	WP_003857896.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
HK406_RS08965	1846964..1847038	-	75	WP_015570794.1	protein YoaJ	-
HK406_RS08970	1847039..1847137	-	99	WP_015570793.1	YoaK family small membrane protein	-
HK406_RS08975	1847183..1848211	-	1029	WP_022650808.1	sensor domain-containing diguanylate cyclase	-
HK406_RS08980	1848515..1848778	+	264	WP_057979958.1	DUF333 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1803374..1849509	46135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T156225 WP_012906750.1 NZ_CP053190:c1843811-1843632 [Enterobacter hormaechei]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T156225 NZ_CP068985:c659841-659467 [Nonomuraea coxensis DSM 45129]
ATGGCGTGGGATATCATCCTCCTAGAACCGGTGGACGTGTGGTTCTTGAAGCTCTGCGAGTCCGACCCGGACACTGCGGC
GCTGATCGAACAGGCCATCGACCGCCTCGCGGAGGTCGGACCCGCGCTGGGGCGTCCGCTCGTTGACACCCTTGAGCACA
GCAACCTCAAGAACTTGAAGGAGCTCCGGCCAGGTTCCCGCAAGAGATCAGAGATCCGAATGCTCTTTGTCTTCGACCCC
GACCGGGCAGCAATCTTCTTGGTCGCTGGTGATAAAGCAGGACAATGGTCCCGATGGTATGACGAGGCCGTACCACTAGC
CGAAGCCCGCTACGCCGATTACCGAGCGGAGCAAGACAAGGAGACCGATCGATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14662.50 Da
• Isoelectric Point: 4.2052

>AT156225 WP_044703865.1 NZ_CP053190:c1843609-1843205 [Enterobacter hormaechei]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Nucleotide

• Length: 405 bp

>AT156225 NZ_CP068985:c659470-659141 [Nonomuraea coxensis DSM 45129]
ATGAGCACCGCCCGCCGATGGCAGGACGTCAAAGCCGAGGCACACCGCCGTCACCCCGAGTTGGCGGAGCCGGAACGTCA
GGCCAAGGCCCGCACCGAACTCGACGCCTACATTGCCGGCCACCACCTCAAGGAACTCCGCAAGTCTACCGGGAAGACCC
AAGCCGAGGTCGCCCAGATCCTGGGTGTTTCCCAGAGCCGGATCTCTCAGATCGAGAACGGGGACATCGAGGCCATGGAA
CTGGAGACCCTCCGCGCCTACGCCGCCGCACTGGGTGGGCATCTCGACATCACCATCAGCGTCGGACCCCACTCGGTCAA
GGTGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4