Detailed information of TA system TA156213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2124380..2124909
• Replicon: chromosome
• Accession: NZ_CP053185
• Organism: Staphylococcus aureus strain Guangzhou-SAU749

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HLG72_RS10545
• Protein ID: WP_000621175.1
• Coordinates: 2124380..2124742 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HLG72_RS10550
• Protein ID: WP_000948331.1
• Coordinates: 2124739..2124909 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG72_RS10525	2121359..2122129	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
HLG72_RS10530	2122104..2122583	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
HLG72_RS10535	2122585..2122911	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HLG72_RS10540	2123030..2124031	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HLG72_RS10545	2124380..2124742	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HLG72_RS10550	2124739..2124909	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HLG72_RS10555	2124994..2126142	-	1149	WP_001280702.1	alanine racemase	-
HLG72_RS10560	2126208..2126567	-	360	WP_000581194.1	holo-ACP synthase	-
HLG72_RS10565	2126571..2127062	-	492	WP_001205915.1	PH domain-containing protein	-
HLG72_RS10570	2127049..2128632	-	1584	WP_001294642.1	PH domain-containing protein	-
HLG72_RS10575	2128625..2129104	-	480	WP_001287083.1	hypothetical protein	-
HLG72_RS10580	2129313..2129873	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T156213 WP_000621175.1 NZ_CP053185:c2124742-2124380 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T156213 NZ_CP068973:c179956-179474 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT156213 WP_000948331.1 NZ_CP053185:c2124909-2124739 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT156213 NZ_CP068973:c180210-179944 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1