Detailed information of TA system TA156191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 60780..61888
• Replicon: plasmid unnamed
• Accession: NZ_CP053182
• Organism: Enterococcus faecalis strain FMAMOB6

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: HLJ13_RS13945
• Protein ID: WP_000233000.1
• Coordinates: 61019..61888 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: HLJ13_RS13940
• Protein ID: WP_000205227.1
• Coordinates: 60780..61004 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLJ13_RS13925 (HLJ13_13905)	56017..56334	-	318	WP_002394802.1	heavy metal-binding domain-containing protein	-
HLJ13_RS13930 (HLJ13_13910)	56937..59909	-	2973	WP_002367800.1	Tn3 family transposase	-
HLJ13_RS13935 (HLJ13_13915)	60040..60645	+	606	WP_002367801.1	recombinase family protein	-
HLJ13_RS13940 (HLJ13_13920)	60780..61004	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
HLJ13_RS13945 (HLJ13_13925)	61019..61888	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
HLJ13_RS13950 (HLJ13_13930)	61869..62603	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
HLJ13_RS13955 (HLJ13_13935)	62636..63544	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
HLJ13_RS13960 (HLJ13_13940)	63541..63768	+	228	Protein_69	hypothetical protein	-
HLJ13_RS13965 (HLJ13_13945)	63883..64791	+	909	WP_002324522.1	IS982-like element ISEfm1 family transposase	-
HLJ13_RS13970 (HLJ13_13950)	64773..65069	+	297	WP_258078154.1	GNAT family N-acetyltransferase	-
HLJ13_RS13975 (HLJ13_13955)	65162..65956	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
HLJ13_RS14060 (HLJ13_13960)	66366..66449	+	84	Protein_73	MLS leader peptide	-
HLJ13_RS13980 (HLJ13_13965)	66498..66581	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(L) / tet(M) / ant(6)-Ia / aph(3')-III / erm(B)	-	1..73942	73942
	-	flank	IS/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	62636..67443	4807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T156191 WP_000233000.1 NZ_CP053182:61019-61888 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T156191 NZ_CP068830:3154541-3155035 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT156191 WP_000205227.1 NZ_CP053182:60780-61004 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT156191 NZ_CP068830:3154251-3154544 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: R3JIN1