Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 52830..53449 Replicon plasmid unnamed
Accession NZ_CP053182
Organism Enterococcus faecalis strain FMAMOB6

Toxin (Protein)


Gene name mazF Uniprot ID R3H4V2
Locus tag HLJ13_RS13890 Protein ID WP_000241511.1
Coordinates 52830..53192 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3H5D1
Locus tag HLJ13_RS13895 Protein ID WP_000245205.1
Coordinates 53186..53449 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLJ13_RS13875 (HLJ13_13855) 48240..50171 - 1932 WP_010816308.1 sucrose-specific PTS transporter subunit IIBC -
HLJ13_RS13880 (HLJ13_13860) 50362..51801 + 1440 WP_002367771.1 sucrose-6-phosphate hydrolase -
HLJ13_RS13885 (HLJ13_13865) 51803..52765 + 963 WP_002367770.1 LacI family DNA-binding transcriptional regulator -
HLJ13_RS13890 (HLJ13_13870) 52830..53192 - 363 WP_000241511.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HLJ13_RS13895 (HLJ13_13875) 53186..53449 - 264 WP_000245205.1 PbsX family transcriptional regulator Antitoxin
HLJ13_RS13900 (HLJ13_13880) 53626..54246 + 621 WP_013438829.1 recombinase family protein -
HLJ13_RS13905 (HLJ13_13885) 54263..54547 + 285 WP_002394798.1 hypothetical protein -
HLJ13_RS13910 (HLJ13_13890) 54549..54782 + 234 WP_002397510.1 hypothetical protein -
HLJ13_RS13915 (HLJ13_13895) 54942..55196 - 255 WP_002394800.1 hypothetical protein -
HLJ13_RS13920 (HLJ13_13900) 55313..55981 - 669 WP_002397511.1 CPBP family intramembrane metalloprotease -
HLJ13_RS13925 (HLJ13_13905) 56017..56334 - 318 WP_002394802.1 heavy metal-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(L) / tet(M) / ant(6)-Ia / aph(3')-III / erm(B) - 1..73942 73942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13585.78 Da        Isoelectric Point: 9.6107

>T156190 WP_000241511.1 NZ_CP053182:c53192-52830 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Download         Length: 363 bp

>T156190 NZ_CP068830:2979009-2979116 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10059.46 Da        Isoelectric Point: 5.1585

>AT156190 WP_000245205.1 NZ_CP053182:c53449-53186 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Download         Length: 264 bp

>AT156190 NZ_CP068830:c2978959-2978896 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3H4V2

Antitoxin

Source ID Structure
AlphaFold DB R3H5D1

References