Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2705210..2705781 Replicon chromosome
Accession NZ_CP053181
Organism Enterococcus faecalis strain FMAMOB6

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag HLJ13_RS13145 Protein ID WP_002360937.1
Coordinates 2705210..2705551 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag HLJ13_RS13150 Protein ID WP_002367500.1
Coordinates 2705551..2705781 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLJ13_RS13140 (2701225) 2701225..2704839 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
HLJ13_RS13145 (2705210) 2705210..2705551 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HLJ13_RS13150 (2705551) 2705551..2705781 - 231 WP_002367500.1 hypothetical protein Antitoxin
HLJ13_RS13155 (2706187) 2706187..2706402 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
HLJ13_RS13160 (2706541) 2706541..2707533 + 993 WP_002398510.1 biotin--[acetyl-CoA-carboxylase] ligase -
HLJ13_RS13165 (2707700) 2707700..2708338 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
HLJ13_RS13170 (2709022) 2709022..2710638 + 1617 WP_002372620.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T156178 WP_002360937.1 NZ_CP053181:c2705551-2705210 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T156178 NZ_CP068830:259369-259476 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT156178 WP_002367500.1 NZ_CP053181:c2705781-2705551 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT156178 NZ_CP068830:c259320-259257 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References