Detailed information of TA system TA156160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4274399..4275033
• Replicon: chromosome
• Accession: NZ_CP053109
• Organism: Streptomyces sp. Z423-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HJD23_RS19085
• Protein ID: WP_171109841.1
• Coordinates: 4274399..4274821 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2T5B9U3
• Locus tag: HJD23_RS19090
• Protein ID: WP_020113663.1
• Coordinates: 4274818..4275033 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJD23_RS19060	4270002..4270748	-	747	WP_171109836.1	phosphatase PAP2 family protein	-
HJD23_RS19065	4270745..4271359	-	615	WP_171109837.1	phosphatase PAP2 family protein	-
HJD23_RS19070	4271368..4271985	-	618	WP_171109838.1	DedA family protein	-
HJD23_RS19075	4272101..4272472	+	372	WP_171109839.1	BlaI/MecI/CopY family transcriptional regulator	-
HJD23_RS19080	4272469..4273398	+	930	WP_171109840.1	M48 family metalloprotease	-
HJD23_RS19085	4274399..4274821	-	423	WP_171109841.1	PIN domain nuclease	Toxin
HJD23_RS19090	4274818..4275033	-	216	WP_020113663.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HJD23_RS19095	4275624..4277636	-	2013	WP_171109842.1	ABC transporter permease	-
HJD23_RS19100	4277633..4278349	-	717	WP_171109843.1	ABC transporter ATP-binding protein	-
HJD23_RS19105	4278512..4279708	+	1197	WP_171109844.1	two-component sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-49)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15425.36 Da
• Isoelectric Point: 4.5746

>T156160 WP_171109841.1 NZ_CP053109:c4274821-4274399 [Streptomyces sp. Z423-1]
VNAAQFLIDTSALARMLRGDAEQYGWDQAAAAGLIATCPITELEFFYSARSPADRAQGIEDMRLLFGWVPVDDRAYDRAW
RVQELLTQRGQHRSAGAVDLVVAATAELQGLTLLHRDRDFECIAAVTGQALQWYGPEAGK

Nucleotide

• Length: 423 bp

>T156160 NZ_CP068829:c2739994-2739818 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7995.02 Da
• Isoelectric Point: 6.3273

>AT156160 WP_020113663.1 NZ_CP053109:c4275033-4274818 [Streptomyces sp. Z423-1]
MSRTVIDLDDQLVADVAKALGTSTKKETVNTALREVLENRRRALALTRLRASAEEGAFDLDLFEDKGNYRR

Nucleotide

• Length: 216 bp

>AT156160 NZ_CP068829:c2739772-2739356 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2T5B9U3