Detailed information of TA system TA156158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 121158..121897
• Replicon: plasmid pEc61A
• Accession: NZ_CP053104
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain Ec61

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A5E1AVR5
• Locus tag: HLA96_RS23975
• Protein ID: WP_013087172.1
• Coordinates: 121158..121643 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A5E1AWX8
• Locus tag: HLA96_RS23980
• Protein ID: WP_012540086.1
• Coordinates: 121631..121897 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLA96_RS23940	116348..116683	-	336	WP_013087184.1	hypothetical protein	-
HLA96_RS23945	116725..117516	-	792	WP_013087183.1	N-6 DNA methylase	-
HLA96_RS23950	117557..117757	-	201	WP_013087182.1	hypothetical protein	-
HLA96_RS23955	117845..118306	-	462	WP_013087181.1	hypothetical protein	-
HLA96_RS23960	118367..118681	-	315	WP_044157807.1	hypothetical protein	-
HLA96_RS23965	119557..119715	-	159	WP_013087178.1	type I toxin-antitoxin system Hok family toxin	-
HLA96_RS23970	119787..120860	+	1074	WP_058672721.1	IS481-like element ISKpn28 family transposase	-
HLA96_RS23975	121158..121643	-	486	WP_013087172.1	GNAT family N-acetyltransferase	Toxin
HLA96_RS23980	121631..121897	-	267	WP_012540086.1	DUF1778 domain-containing protein	Antitoxin
HLA96_RS23985	122197..122520	+	324	WP_013087171.1	metalloregulator ArsR/SmtB family transcription factor	-
HLA96_RS23990	122522..123235	+	714	WP_013087170.1	arsenical resistance protein ArsH	-
HLA96_RS23995	123244..123789	+	546	WP_013087169.1	sigma-70 family RNA polymerase sigma factor	-
HLA96_RS24000	123865..124227	+	363	WP_013087168.1	arsenic metallochaperone ArsD family protein	-
HLA96_RS24005	124254..126005	+	1752	WP_162875198.1	arsenical pump-driving ATPase	-
HLA96_RS24010	126130..126666	+	537	WP_013087166.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-9	-	1..149802	149802
	-	flank	IS/Tn	-	-	119787..120860	1073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17810.62 Da
• Isoelectric Point: 9.8560

>T156158 WP_013087172.1 NZ_CP053104:c121643-121158 [Enterobacter hormaechei subsp. xiangfangensis]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T156158 NZ_CP068829:2280596-2280699 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10076.47 Da
• Isoelectric Point: 4.3721

>AT156158 WP_012540086.1 NZ_CP053104:c121897-121631 [Enterobacter hormaechei subsp. xiangfangensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT156158 NZ_CP068829:c2280737-2280466 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A5E1AVR5

Antitoxin

• AlphaFold DB: A0A5E1AWX8