Detailed information of TA system TA156157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 11592..12331
• Replicon: plasmid pEc61A
• Accession: NZ_CP053104
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain Ec61

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A9E6WPH7
• Locus tag: HLA96_RS23375
• Protein ID: WP_013087269.1
• Coordinates: 11846..12331 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A9E6WPN8
• Locus tag: HLA96_RS23370
• Protein ID: WP_007869691.1
• Coordinates: 11592..11858 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLA96_RS23335	8116..8523	-	408	WP_000522993.1	organomercurial transporter MerC	-
HLA96_RS23340	8551..8826	-	276	WP_000732276.1	mercury resistance system periplasmic binding protein MerP	-
HLA96_RS23345	8842..9192	-	351	WP_197620796.1	mercuric ion transporter MerT	-
HLA96_RS23350	9264..9719	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
HLA96_RS23355	9752..9925	-	174	Protein_16	DUF4158 domain-containing protein	-
HLA96_RS23360	10082..10786	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HLA96_RS23365	10839..11468	+	630	Protein_18	Tn3 family transposase	-
HLA96_RS23370	11592..11858	+	267	WP_007869691.1	DUF1778 domain-containing protein	Antitoxin
HLA96_RS23375	11846..12331	+	486	WP_013087269.1	GNAT family N-acetyltransferase	Toxin
HLA96_RS23380	12509..12739	-	231	WP_001572393.1	hypothetical protein	-
HLA96_RS23385	13397..13630	-	234	WP_015059496.1	hypothetical protein	-
HLA96_RS23390	13852..14748	-	897	WP_197620802.1	hypothetical protein	-
HLA96_RS23395	14751..15266	-	516	WP_001572389.1	thermonuclease family protein	-
HLA96_RS23400	15481..16659	+	1179	Protein_25	DNA (cytosine-5-)-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-9	-	1..149802	149802

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17736.45 Da
• Isoelectric Point: 9.5181

>T156157 WP_013087269.1 NZ_CP053104:11846-12331 [Enterobacter hormaechei subsp. xiangfangensis]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T156157 NZ_CP068829:1226210-1226521 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10080.46 Da
• Isoelectric Point: 4.3837

>AT156157 WP_007869691.1 NZ_CP053104:11592-11858 [Enterobacter hormaechei subsp. xiangfangensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT156157 NZ_CP068829:1226518-1226937 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA