Detailed information of TA system TA156089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 5480..6068
• Replicon: plasmid unnamed2
• Accession: NZ_CP053100
• Organism: Acinetobacter baumannii ATCC 17978

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: HKO16_RS19230
• Protein ID: WP_000438826.1
• Coordinates: 5480..5767 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: HKO16_RS19235
• Protein ID: WP_061855757.1
• Coordinates: 5754..6068 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKO16_RS19195	944..1519	+	576	WP_001096616.1	helix-turn-helix domain-containing protein	-
HKO16_RS19200	1539..1682	+	144	WP_001125244.1	hypothetical protein	-
HKO16_RS19205	1867..2070	-	204	WP_000482656.1	hypothetical protein	-
HKO16_RS19210	2314..2559	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HKO16_RS19215	2549..2842	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HKO16_RS19220	3060..4262	+	1203	WP_061855756.1	alpha/beta fold hydrolase	-
HKO16_RS19225	4360..5133	+	774	WP_061855758.1	glucose 1-dehydrogenase	-
HKO16_RS19230	5480..5767	+	288	WP_000438826.1	BrnT family toxin	Toxin
HKO16_RS19235	5754..6068	+	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
HKO16_RS19240	6197..8608	+	2412	WP_000932945.1	TonB-dependent receptor ZnuD2	-
HKO16_RS19245	8705..9403	+	699	WP_000007662.1	CPBP family intramembrane metalloprotease	-
HKO16_RS19250	9525..9722	-	198	WP_001282483.1	hypothetical protein	-
HKO16_RS19255	9722..10093	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11302	11302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T156089 WP_000438826.1 NZ_CP053100:5480-5767 [Acinetobacter baumannii ATCC 17978]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T156089 NZ_CP068826:4446571-4446828 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGCCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT156089 WP_061855757.1 NZ_CP053100:5754-6068 [Acinetobacter baumannii ATCC 17978]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT156089 NZ_CP068826:4446840-4447391 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: A0A858UV00