Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2671829..2672480 Replicon chromosome
Accession NZ_CP053093
Organism Lacticaseibacillus paracasei strain CLP-Y5

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag HLG73_RS13175 Protein ID WP_003567661.1
Coordinates 2671829..2672212 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag HLG73_RS13180 Protein ID WP_003567665.1
Coordinates 2672232..2672480 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLG73_RS13170 (HLG73_13330) 2671134..2671655 - 522 WP_012492007.1 QueT transporter family protein -
HLG73_RS13175 (HLG73_13335) 2671829..2672212 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HLG73_RS13180 (HLG73_13340) 2672232..2672480 - 249 WP_003567665.1 antitoxin Antitoxin
HLG73_RS13185 (HLG73_13345) 2672548..2673684 - 1137 WP_003576478.1 alanine racemase -
HLG73_RS13190 (HLG73_13350) 2673671..2674045 - 375 WP_003567669.1 holo-ACP synthase -
HLG73_RS13195 (HLG73_13355) 2674215..2675723 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
HLG73_RS13200 (HLG73_13360) 2675849..2675992 - 144 WP_003588978.1 hypothetical protein -
HLG73_RS13205 (HLG73_13365) 2676023..2677411 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T156082 WP_003567661.1 NZ_CP053093:c2672212-2671829 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T156082 NZ_CP068826:2956703-2956810 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT156082 WP_003567665.1 NZ_CP053093:c2672480-2672232 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT156082 NZ_CP068826:c2956653-2956590 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References