Detailed information of TA system TA156080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 443881..444509
• Replicon: chromosome
• Accession: NZ_CP053093
• Organism: Lacticaseibacillus paracasei strain CLP-Y5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S2NVS0
• Locus tag: HLG73_RS02180
• Protein ID: WP_016373562.1
• Coordinates: 444141..444509 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: HLG73_RS02175
• Protein ID: WP_003583264.1
• Coordinates: 443881..444141 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG73_RS02145 (HLG73_02155)	439764..440294	+	531	WP_165846938.1	DNA-binding response regulator	-
HLG73_RS02150 (HLG73_02160)	440369..440521	+	153	WP_003563363.1	YvrJ family protein	-
HLG73_RS02155 (HLG73_02165)	440531..441295	+	765	WP_273634556.1	GH25 family lysozyme	-
HLG73_RS02160 (HLG73_02170)	441506..442009	+	504	WP_003583259.1	TetR/AcrR family transcriptional regulator	-
HLG73_RS02165 (HLG73_02175)	442014..443075	+	1062	WP_273634557.1	ABC transporter permease	-
HLG73_RS02170 (HLG73_02180)	443080..443769	+	690	WP_003563376.1	ABC transporter ATP-binding protein	-
HLG73_RS02175 (HLG73_02185)	443881..444141	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
HLG73_RS02180 (HLG73_02190)	444141..444509	-	369	WP_016373562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLG73_RS02185 (HLG73_02195)	444801..446171	-	1371	WP_003573580.1	FAD-dependent oxidoreductase	-
HLG73_RS02190 (HLG73_02200)	446535..447353	-	819	WP_273634558.1	Cof-type HAD-IIB family hydrolase	-
HLG73_RS02195 (HLG73_02205)	447668..447937	-	270	WP_003563382.1	type II toxin-antitoxin system YafQ family toxin	-
HLG73_RS02200 (HLG73_02210)	448178..449185	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	434663..444509	9846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14923.01 Da
• Isoelectric Point: 8.3986

>T156080 WP_016373562.1 NZ_CP053093:c444509-444141 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMPERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQANLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T156080 NZ_CP053093:c444509-444141 [Lacticaseibacillus paracasei]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCCAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGGCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT156080 WP_003583264.1 NZ_CP053093:c444141-443881 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT156080 NZ_CP053093:c444141-443881 [Lacticaseibacillus paracasei]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: S2NVS0