Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3056936..3057623 Replicon chromosome
Accession NZ_CP053092
Organism Mycobacterium tuberculosis strain 4860

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag HGO90_RS14445 Protein ID WP_003414064.1
Coordinates 3057228..3057623 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag HGO90_RS14440 Protein ID WP_003414061.1
Coordinates 3056936..3057202 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGO90_RS14415 3052575..3053477 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
HGO90_RS14420 3053546..3054298 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
HGO90_RS14425 3054542..3054817 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
HGO90_RS14430 3054814..3056436 - 1623 WP_003906897.1 type I restriction-modification system subunit M -
HGO90_RS14435 3056523..3056939 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
HGO90_RS14440 3056936..3057202 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HGO90_RS14445 3057228..3057623 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
HGO90_RS14450 3057620..3057889 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
HGO90_RS14455 3057899..3058993 - 1095 WP_003414068.1 restriction endonuclease subunit S -
HGO90_RS14460 3058990..3059409 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
HGO90_RS14465 3059483..3059962 - 480 WP_003414073.1 dihydrofolate reductase -
HGO90_RS14470 3060033..3060833 - 801 WP_009939104.1 thymidylate synthase -
HGO90_RS14475 3060989..3061726 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T156069 WP_003414064.1 NZ_CP053092:c3057623-3057228 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T156069 NZ_CP068826:216762-216869 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT156069 WP_003414061.1 NZ_CP053092:c3057202-3056936 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT156069 NZ_CP068826:c216704-216650 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TF65

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References