Detailed information of TA system TA156068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3056523..3057202
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: HGO90_RS14435
• Protein ID: WP_003414059.1
• Coordinates: 3056523..3056939 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: HGO90_RS14440
• Protein ID: WP_003414061.1
• Coordinates: 3056936..3057202 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS14415	3052575..3053477	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HGO90_RS14420	3053546..3054298	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
HGO90_RS14425	3054542..3054817	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
HGO90_RS14430	3054814..3056436	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
HGO90_RS14435	3056523..3056939	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
HGO90_RS14440	3056936..3057202	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HGO90_RS14445	3057228..3057623	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
HGO90_RS14450	3057620..3057889	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
HGO90_RS14455	3057899..3058993	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
HGO90_RS14460	3058990..3059409	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
HGO90_RS14465	3059483..3059962	-	480	WP_003414073.1	dihydrofolate reductase	-
HGO90_RS14470	3060033..3060833	-	801	WP_009939104.1	thymidylate synthase	-
HGO90_RS14475	3060989..3061726	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T156068 WP_003414059.1 NZ_CP053092:c3056939-3056523 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T156068 NZ_CP068826:161245-161430 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT156068 WP_003414061.1 NZ_CP053092:c3057202-3056936 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT156068 NZ_CP068826:161447-161857 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64