Detailed information of TA system TA156064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2856161..2856792
• Replicon: chromosome
• Accession: NZ_CP053092
• Organism: Mycobacterium tuberculosis strain 4860

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: HGO90_RS13350
• Protein ID: WP_003413174.1
• Coordinates: 2856415..2856792 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: HGO90_RS13345
• Protein ID: WP_003413167.1
• Coordinates: 2856161..2856418 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO90_RS13315	2852075..2852389	+	315	WP_009937839.1	hypothetical protein	-
HGO90_RS13320	2852685..2853896	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
HGO90_RS13325	2854023..2854682	+	660	WP_014585545.1	LppA family lipoprotein	-
HGO90_RS13330	2854679..2855341	+	663	WP_003900848.1	hypothetical protein	-
HGO90_RS13335	2855338..2855616	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
HGO90_RS13340	2855709..2856122	+	414	WP_003413164.1	PIN domain nuclease	-
HGO90_RS13345	2856161..2856418	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
HGO90_RS13350	2856415..2856792	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
HGO90_RS13355	2856808..2857182	-	375	WP_003413177.1	hypothetical protein	-
HGO90_RS13360	2857282..2857677	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
HGO90_RS13365	2857674..2857919	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
HGO90_RS13370	2858330..2858749	-	420	WP_003413190.1	A24 family peptidase	-
HGO90_RS13375	2858761..2859570	-	810	WP_003413193.1	shikimate dehydrogenase	-
HGO90_RS13380	2859567..2860820	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
HGO90_RS13385	2860813..2861325	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T156064 WP_003413174.1 NZ_CP053092:2856415-2856792 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T156064 NZ_CP068825:c4115475-4115179 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT156064 WP_003413167.1 NZ_CP053092:2856161-2856418 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT156064 NZ_CP068825:c4115177-4114782 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5